Saturday, July 12, 2008
the methods shown below can be used for detection of aflatoxins in milk thou grain was used as the main food for the mentioned methods.
Minicolumn
Finely ground grain is extracted with solvents, purified by a precipitation procedure, and the extract washed through a column containing two absorbents. Migration and long wave UV light are used for detection.
Rapid (9 to 15 min.), simple, and semi-qualitative; requires inexpensive equipment; can detect aflatoxins down to 4 ppb.
Quick but only qualitative. Can be used as a “go” or “no go” measurement above 4 ppb. The short minicolumn test is not suited for mixed feeds.
Enzyme Linked Immunosorbent Assay or “ELISA”
Grain is extracted in methanol and placed in plastic well. Addition of antibody-enzyme conjugate and chromagen results in color which is quantitative measure of alkaloid.
Test is specific for target alkaloid but may be crossreactive within members of an alkaloid group. Sensitive to 5 ppb (aflatoxin) and requires 10 minutes to complete.
ELISA requires a plate reader for accurate quantitation, but no other specialized equipment is necessary. ELISA is a good compromise of sensitivity, speed and expense.
Immunoaffinity column assay (ICA)
* similar to ELISA
http://diagnostics.montana.edu/GPDN/Mycotoxin/MYCOTOXINS.pdf
Applied Food Science And Nutrition.
11:26 PM
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